| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Clostridium tetani | CGGAGCAGGAATGGTAATGTC | ACAGGCCATTTCTCCTCCAG | 58.79 | 59.38 | 259 | 158 |
100.00%
|
100.00%
|
| Primer 2 | Clostridium tetani | CGGAGCAGGAATGGTAATGTC | TACAGGCCATTTCTCCTCCAG | 58.79 | 59.16 | 260 | 158 |
100.00%
|
100.00%
|
| Primer 3 | Clostridium tetani | AAACGGCTCAGGTGTCAGC | AACTTGAACACCATCTGCTGT | 60.60 | 58.06 | 224 | 148 |
100.00%
|
100.00%
|
| Primer 4 | Clostridium tetani | TGAGAAAACGGCTCAGGTGT | AACTTGAACACCATCTGCTGT | 59.53 | 58.06 | 229 | 148 |
100.00%
|
100.00%
|
| Primer 5 | Clostridium tetani | TGCGGAACTTGTGCCTGT | TGGCACGCATCATCACATC | 59.81 | 58.60 | 248 | 148 |
100.00%
|
100.00%
|
| Primer 6 | Clostridium tetani | TGCGGAACTTGTGCCTGT | TCTTGGCACGCATCATCAC | 59.81 | 58.53 | 251 | 148 |
100.00%
|
100.00%
|
| Primer 7 | Clostridium tetani | TGCGGAACTTGTGCCTGT | TTGGCACGCATCATCACATC | 59.81 | 59.27 | 249 | 148 |
100.00%
|
100.00%
|
| Primer 8 | Clostridium tetani | TGCGGAACTTGTGCCTGT | CTTGGCACGCATCATCACAT | 59.81 | 59.26 | 250 | 148 |
100.00%
|
100.00%
|
| Primer 9 | Clostridium tetani | TGCGGAACTTGTGCCTGT | TTCTTGGCACGCATCATCAC | 59.81 | 59.20 | 252 | 148 |
100.00%
|
100.00%
|
| Primer 10 | Clostridium tetani | TGCGGAACTTGTGCCTGT | TTGGCACGCATCATCACAT | 59.81 | 58.13 | 249 | 148 |
100.00%
|
100.00%
|